	   SnapGene      ”tgctttctctgaccagcattctctcccctgggcctgtgccgctttctgtctgcagcttgtggcctgggtcacctctacggctggcccagatccttccctgccgcctccttcaggttccgtcttcctccactccctcttccccttgctctctgctgtgttgctgcccaaggatgctctttccggagcacttccttctcggcgctgcaccacgtgatgtcctctgagcggatcctccccgtgtctgggtcctctccgggcatctctcctccctcacccaaccccatgccgtcttcactcgctgggttcccttttccttctccttctggggcctgtgccatctctcgtttcttaggatggccttctccgacggatgtctcccttgcgtcccgcctccccttcttgtaggcctgcatcatcaccgtttttctggacaaccccaaagtaccccgtctccctggctttagccacctctccatcctcttgctttctttgcctggacaccccgttctcctgtggattcgggtcacctctcactcctttcatttgggcagctcccctaccccccttacctctctagtctgtgctagctcttccagccccctgtcatggcatcttccaggggtccgagagctcagctagtcttcttcctccaacccgggcccctatgtccacttcaggacagcatgtttgctgcctccagggatcctgtgtccccgagctgggaccaccttatattcccagggccggttaatgtggctctggttctgggtacttttatctgtcccctccaccccacagtggggcaataacttcgtatagcatacattatacgaagttataagcttctgacctcttctcttcctcccacagggcctcgagagatctggcagcggagagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctaggctcgagatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgatctagagggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgggtctcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcggggagtcgctgcgacgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaatgtcgaccgccaccatgATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCCAAGATCCATTCGTTGGGGGATCCGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGtgacgcgtgaattcactcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgcagcccaagcttataacttcgtatagcatacattatacgaagttatgcggccgcaccggtatgcatttaattaagatatcagctttactagggacaggattggtgacagaaaagccccatccttaggcctcctccttcctagtctcctgatattgggtctaacccccacctcctgttaggcagattccttatctggtgacacacccccatttcctggagccatctctctccttgccagaacctctaaggtttgcttacgatggagccagagaggatcctgggagggagagcttggcagggggtgggagggaagggggggatgcgtgacctgcccggttctcagtggccaccctgcgctaccctctcccagaacctgagctgctctgacgcggctgtctggtgcgtttcactgatcctggtgctgcagcttccttacacttcccaagaggagaagcagtttggaaaaacaaaatcagaataagttggtcctgagttctaactttggctcttcacctttctagtccccaatttatattgttcctccgtgcgtcagttttacctgtgagataaggccagtagccagccccgtcctggcagggctgtggtgaggaggggggtgtccgtgtggaaaactccctttgtgagaatggtgcgtcctaggtgttcaccaggtcgtggccgcctctactccctttctctttctccatccttctttccttaaagagtccccagtgctatctgggacatattcctccgcccagagcagggtcccgcttccctaaggccctgctctgggcttctgggtttgagtccttggcaagcccaggagaggcgctcaggcttccctgtcccccttcctcgtccaccatctcatgcccctggctctcctgccccttccctacaggggttcctggctctgctctaagggcgaattcgccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttgaattcgccctt  A)   j   &   !   G   (            8            J   )   6   /   ,         *   $               	                     %         7   
                     "            #   H      !   7   +            <         2   6   )   &   ?   -   !                                  !         "   $               (                                                                                                      
                        '   (      "   2            %   &            %      &   2      	                                                               "            3   $         2   1                           .      )   2   "      E   7   9         C   &      
      %   	         -         A   $         )   "                     )            .   <       $   0                     '   B         5   G   *         F   &      "   +   :      9   8   2   0   ;   Y        j Dj¸¹‹ÈÉÊÏÔãäH						
										#	(	-	4	9	>	²	q
Š
‹
Œ
˜
™
š
³
ï
/0123=Q^_`akl¾ÊÖéêëúû'(-Ð÷.:;<LMNOU_HImì�ijxyz{£¤¥Çnª& »ÐåîÐg³	
y
�
´
>)‡¯ø=PJû‘ÿšW[os|$¸“cm:! A ó ,kþB~WŒËÐIæ›
Rbm¸ìü`nÚk¦=aÈo‚G  þ E!�ä e€ñhºQ]Õ		$	)	.	5	:	?	®	
/
r
}
ä
ð
4s€¿Ë×å!.€ÊÑóü/V[³Z±·ªËí”YÝ gÃ«B( µ {¡¤©!sxœæcêH	´	z
Ž
µ
*>¢°ù`kÚøè /EX\pÿ yd ‡wžï?éí¾Üót²ëq¹î¿œ#;K ¨ â _p¼éKü P}žwN”¯n» ¬ï¤Ñ1…ŸëÚÑh
ˆ>Q‡€ÝU’÷›%SÂ8 ™Ñy”¾�áø{¸ÌÑç¡M	
œ
 *Scn¹í÷ýÚQorž¶72GJQ¥lÎá›§ÈÓBè‘§!H$ ÿ{õ<qyÛ1Kp†Íú[ p -éøæ,A
a¿%V§7ŽbÉ$p¥äƒ B ô 	lCšXJ›|~ßeÛ$Þ>îp›÷Ô²J Å Œ—»={		 	%	*	/	6	;	@	E	R	e		„	‡	Š	�	�	«	¯	
Ò
å
ê
tÌÑæð	/4FRW�³ËÒßô\mx#[‹¬²¸«†~ÞEdÁŽ€Lb¬C) ÿ "_!u�tR^ÖýLvU	óåW´ØÇè‡Ìáî/Y1òe�oc	@ê'6 N ÈòµiíÂÇ‹õ	0
L
^
s
“
Ã
Õ
ñ
5G�²ÀØ"I0Bf{&`8Mb´FZÆ7•Z†°Ðµh�`/  < R Fc”‘åf®òÎÙñ3~‚C“	!
~
­7ý—ûŒVp½˜zäNøªtÄÏ, ~…¢yßœçŸ2	C	K	‚	o
{
;+P~±ú^¯µœ¨ÛSc¥NYqýž¡|@„e qln|¥ºªÈ�I	|Îs0L] sÂz¡�=Ë ÃÝt³dëµ	Ÿùé+*g@šõíƒ'S* ¶ Þ sË>J¬â"‚¾¤a	
�
¶
Û
f§?Z£‚Ta‚lÃ¬¾¥F_·8#€È$ ?ˆ³èxð¢	ÕêÕ;]¡È(ÐD›ôfurº¢¿ÞWÐÀÏÅ$u Ÿo	f
¨î¿s–¬³HïF„�¯L §Þ$ÝÄá?<T‡ S@¹+:yO-±ì€… © Ô `q¼ þñ»B~×	 ŠÔu]ŸU^…o ê&¬ù¡öx;•£¤ X ã ½p,Ê.<LC×ýu2QÛOZb°¼%zŒº¼Íñ mœäç†‰Ù¨´Ý09¿	
I
?RýˆŽ�„ÞV‰Á 3u ­ð¥nÛó ® ž“øœ¨&OPÃüF 8-Òiû	‰EÒ­ TUE  
 ÒO¿2 IŸìÿq�š_ÿ	tåÏ3’kÅñ:…Ž�O% :  ðŸ|•*vÍÒè\
±
\qÏîøpHKTm–™°âC¿¥¨"}ÒF Ò š¢
d
Ù
drRŸ·8ï"¦ƒqœÀ% š í Ãz¿¹¥
§
©
«
TVop¦RÕ×æÂ¨®ÔéPIÖD7 # KDÒjŽâù<f|÷N	Ð	†
�
¡
­
¿
÷
!+MXºàþxžÛºÆ.sô _’3Ïâ`É}’{™�å
 ‘µØë‹ûR <|Äö=4
r‘ñ2LqïÈÚ -·í„ÝmTzÜ‡ÇÎå\F ¹ Åö	"YzÆû) œ ßFT¡EÙW!þ÷>ü.1–¾ÌUÈQ„ êB2m
À¨ÄiîåŽ …¦Øå W¤­!Ws	B
Q
&-½ì8Óhcg%-Ó#W" q .øù
Áç-Rf»­b“fŸØ¤ë�,XÖÊ‰äòœ3q£Ó )e€°:gÜœ·	y}MâßïMT C 
ZDÐ»K«à3Šfd<œKÁ5e / v ï tÀ@~‹›@Y¯ËèÍÜÊ;qø*ñ# × õ mšÅêŠ“4ôø¨Pfµàƒ%H‹ªÍ?i�œ¦BÕ³H à y—@ƒ’˜¶¼ä|À	!	&	+	7	<	F	P	S	c	…	ˆ	‹	Ž	‘	©	¬	°	

ˆ
‘
Á
Ó
â
è
í
(-O¼Ôçñ
25GU ÈÎØÝõ8]y$nÄ�£J âü0	A	€	 	½		9XzÓ\Œ­³¹¬‡ÕßF‚#‹�M­D! }c®(>k
b
Ÿ
£
¯
½
KZwÍn¤] �ÑbËüe½c7 Æ Ôa�Óõb„†¦ð			f	h	¥	§	ç	;
¸
É
Ý
æ
ë
ù
uÒ0AS‚´Ìà35n—Ó¡Â&ÔAÊ'x+ 3 #v‚÷_×úwùV	 hô_„æX>%Zav5?ófç‘:dg}58CÌÓë‚ "OuúS­ß…µÙVÈéˆâÀÏ2p
1€­Ú)›(2   ™`©2Õû¥ªþM¾Í0§Y~%Aóùx ¸ Ë úò‘©¼àApï®t	îç< O — Ÿ È )±ÍLî+Œº	Â	1
_
Ë
Ö
ß
6H‚¤J1C¼È	c„ÌíÅõ”—å9NCG€¹Çä–Õý#Û}Å Fz�Š+aµ[£”¶:  ( 6 ÉâIÈ²=
M
ò
³g"H‘<aêD[‡—Ä	ia2 � ¬ &Juó¶jlÃj	×	ö	t
”
Ä
û
ÁÙ#|­'dc¼8T�±ÑÖ6u�ÏöÖþx–žPkªX’6 % c æó�¦ÅÚòdˆÖÜ	ß	é	º
Ýâ C\¥¶¹â 0(™hõøqåù”¼ÅÿÐ/) MÏÛ!64IM~É	Y	bé7˜¾T	HW„Jõ)H™{‚ç]já¸4z&  = f Gd„•ë´gÖ”3>h‡HÅ	â	j
hþ)F„™<›¾èª(«Aæ? S ¢ S’¯ËÏ$E�¨ä[Yƒ“¡ý&DT¦”	Ë	Ò	ì	ð	"

Î
#®8uðöüY�š¹]ž;yB±Š†3Otª¹u^�- iºãdðPVâÏ3	p
|
<j ,†ûZY°¶©Ê™Zhrw“¢<ÆÜ’fÂ9A�! § ^£†£ ê�Ùèèì_jÙÜT½öŸOþŸxêRvÁM®º" o xàz· L	
ö�61FUd¦6ÒÝAÚí… “´zü	D	ƒ	FQ²_7¼ñyM}…÷c�?& rm}„«J	`	}�*bKM^ü7{?™ô
 ¦RÝ'*xÏC•«,°Ã¾EVÎÄ>„ W o»Éÿ;Bt«ø1‰õt\:Y¢»T£Ì žò‘ÄOŽ u-Ô•¤|zÑÕC� ¸Ä´;Ãì!Xyúl~ÆäîÇ(  AÞE1Qer	 ž×£ê,h +gã›äöÒÿ="„ËTÒ ?}Š‰¶	O´ÌGh;Að4! ß x?­µãaƒ¿ûb	Ÿ	¤	
:
�
·
Ü
@Ç×£m\Ò¼�"É¢  · ¨1ùÞùg¨U=@$u­¿¦X9$f|47¬í�øw 5 %ItyÌHK¬bƒËìÄôcG�`¸étÎüýj©" ÎRÊ#2LX¥X	i
[a¤(ƒ'�xšôA°G�…sià·»$ ¦ wUéyáEëX^~ÉØ5E¼õT5vxLÛöÀL‘­Á~¹%8@ QñÜ:�)AÃaˆû[NóUX ·@‰ q	�ÖN¢g:£ÑãÐÆÊ3Ž GÉ´ž	£	h
ÖÊë<ó#Ñœ™F¯rs»ûß3¡v ¶p	�	g
DÛÂ»S`˜®õZòK¬â �7( —›	œ	‡ˆ—Ü­´óô,-ðñGHIJ…ª«ÊË�žŸŒž°*456 ˜ ~?˜©ïi‰ÀÝ.ÅÌØ+M %ø“tµI†ñ²Ÿ± í™±ã6@F
ðßZÅÍ‚,N $”ö8®Ìj¸Ñ¯@hr=Ul ¡B×î6
%ŠÁÞÞ„NâÆø÷ˆ ¨>\	t™ª/ÙÝ —Æ·”,Ë�+w  ’üAäMz4PuÓs 'TtùŠÿ;N1¶4¯‡òXÜ†²í °‹¯&‡�º¡à.²íJF³/Ü a]×a€äAe™ÿ}	oñÝ
Žý-CÆ^âè ½ýš²7]G
ï9™MqŸÎëƒŠ Õ r˜«î¹‘àòê‰”ØO ª • 	R—¼W[9Al|¨H- /÷9VÁÍÒ“VÌ; A %E•r¯™	¹^LvXV°õp> Û]_û7?o¶‹ÕS)çém 4;k[b^ _†isV\ ¢¬ë#*¥Ú-eÃÌ¢×Uù7™r 8'5CNO†“ï7
‹_…y<¤Çõo ïØßãçòÓÂÆß!­¸Çßú€OÉ–.¥z  Ý¾ÃA&‡Ž÷+ãfø8‰ ;qË§�uÀÏPƒ08ZC» ›ž/?]	Ð2Øþ@–	i�'§Ú©{Û–~ PbqP~Àš«Üuv©þ £ÞÐPc±Þ&p½òû Y ä Ú¾©-=MyçDÖ3¶RÜ-[½7âì�Î L nÏŠFyó:
J
«È@S‰•Å…ß-WŠ³lakI =å‡¼˜C‚Ìd@!S‚\x Ð �èÚ©µÞ1‚«¸�dŒ,¡ ø_¤Ç$À	þÂ4Ó?v”N ¦býÜ
ªv�©ÄPt—Äý=  ®ÇôB›Žñý!KŸgQ* 6“9o!p[|7åNÛ”ù5Ôè]'ˆ}
 ñ$¯{òQ¹G 9‡úü	pØ`mð !î .‹ o¥ñ–U5°»Y º ž(uÓjrŠFÓ<®¡Oô2VóÝ‡ € %U�·
*ï·îˆFÞ+³áX^ ¡À&3G7ÍÓí� 
uæjå»òvî› ˆšPuÂ  Í Ó¡JM–Žìâ	4rlr¢mÚ `m´0 F Ò7ÖP °ô`Á¨;ç/†³?m¢è·' ±»ó.ŒÞü 'r›Ñˆ“¾Ð .“±}°Æá
œ¦K�G�Ýé(  @ ò +C Ïý}\ÇÎÓíåf 	²
]rù®ò²Gë�þÙVn#X`ÿ «î žw›vbé†÷çÛ.D±p~›" ÷+÷š@
p¾q}µÞIP$¤Íà#šÇo¦Ìù#šöÓGZã#2 ; Q bÇ^ñt–Øð2�ŠBKu~	]
Ðïl×úLUa³0EYd—6�ãn¯ÏDÀb©s_Îé Ý á›²£
n
Ú
e¦S«½r¤¤¶)r�Áf? ì&<Ê e
Ô: ¸Ç9#šNr¡ÝÏSt† Ó -%ðÓÚa„‹ð% 	 N›¾þp,Ž§³Ü/8^ú	þ	H
süÎð¬Ÿ
§„ÀtûD& " 9 ™ JÿCÂï{;ö[
…
¦
¨
ª
¬
Ø
ö
UWp�qÅSÖåÁp˜¯¿˜ �Ü3
×êŠQEÙ › ¬ÀSVºP
’eØ¼ÃëíWÕˆ,û0•×ÇP . Ö î sY™Ä‰®ºL’3Êáó§ç‚É©Ž¥)JÀ% |–Ó
O	¼	a
‡
ž
¢
®
À
á
ø
,8JNY»yŸÜm€ ÐaÊ~%Ó&I 2 Ê ˜Nú¤ø„�ß`4Î>}æ0Ùšòæ&   � – ž « áE°k±£‰»Ç!“–äB;»ãSÕ “Ú|9W‘2 $ b ¡ Lê³�ã=HZg}üSÄ	Ñ	Í
"áÿgE˜/t½ïõX™;\ƒ:I(‰©¸“@þ3]. ]’³¶Î˜»ðÑÙìŒ	 €)”ª½ºS	 >|D0:PÂˆÓËF<!B ÍH0øW9
ÆóW€Í{ü¨€ F‡4wKÚM�É? $–}÷>š	’º_ÛòYÄÉ×áð±‹ ì“7=°í5
E
s·ƒ÷öq &‘sÅŒÒMß03r®WÛ —`¸Qd|	n�ü8@p,k{ˆê]çG Ú.:D^˜	]nµKUaRWæè ÜÂ)4î,…’ÞâæñÂÅ �·ÆÖT*È-ônq ?ŒýÝ,7YÏ¦¨áÚ} K <÷»Exò#�ÇÄcc?²>k[`j 5Æo
Z{©�üç\ñÃ–¸<‡ q
ð•íÿ*W] º-6•-q§l¥l_š¡çl‡tÁ ìt?
ÖFÚ#/X£Øc
"5mËø™õYÍš ào$+¦Û.m
£ž£e> `ÉRs… 8 �U­ÛõO
„
õ
‘Ää»ÀV¾+ú/—¿ Ž � {àé¯GY£¢fÍ':ÿ/?ý%28V\ \ÌO‡8
(©ºïz§ ë#6ìD
Ñ‚†�ÃÈÖöŠ" Ù9C(3Pcáåð{	mÁŒÄŒ´Å+J`jüQ+X¥§àÙæp| 5Dnwñ”
æï”>ðk™æ=k†þÀ  è®XÚôƒ
ô
�Õã".º¿9	4½dÊ*._˜$X Ë"ðàäëz	C
lÃ'³Ðî�“PjÂÕ¦ß‰ÿ{3 4¥¦Ùèéêóy	V
k¢£ÔyzÇàáâ®¯°±²¹¾ÈÉÊËàíûüi	&'()f—ÁÔþfg$  * ° 5X§äŽÕlt	R
W
¤{ÈÌáýÔBôú
h­Æg/h *ÂÈ	µIcB¥ìQäµåóë0¯§ÔŠ l " ÊS´”g Ù$K{Õ>'ÉÕ<×´4cgõi2   + À :@YgŒ¨åù�úKUŽ¸A¿Ä®&
S
X
iœŸHx°Í&�-Cõû]®Ç�hù9¤1 ] r y ˆ ± ¼ /6Ne„ÞZYè.—k�g¼Ömu	Œ¥|ˆ�âþÇøìöY	,F‰–¾ËiŠrb Ã frÌ;Å–
z~¶%,Jd„ñ¶æì°¨ j¸*<l±Ý6�Nã€¦ÁÐQ81Õ U+hvCíRàš{ð91NDRU¼ã 'Ù �ÃC¨Ž¸	É	å	Û	fåô[‹ m w¡0^	¡%/AjŽ“eƒ=áz ªhÂö @¬á4L|Ö3?8S¨Û|-f·j œ[¼LT‹•ÎÑÚ—Ê
g¦Ê�5dà6”—Z D #ŸE]ËÑµhÙÿ(eíÖ=Ø(ÑŠµ˜LÜ.  , 0 ” Û ð A©Aõû	LrœZQ°Å¯ø	Y
›¬j‡Ý�ª±ÿ¾Ë!V^ÓÑØrù'q: I <�¹BÀÄ~Ý2.DëØiß+ò) w † ®  Z�ÞæLc‚SXŒWT
� IvywÎ$):ø<ž d²üZ¥¾óü`2    ’ Á ù ;QShuÁÕú:VA¹Á'
#Ìéë'Î "‘ªöü¤³ƒ¯ÈßQúµ" s ² û jn.£hl	Ù	,
v
Æ
ý
Ã5õN©QE¶]«0‹§.ãm· } ×Ofˆ›·×%>g„�þ�øC8 éíÏE Z i z ½ Ø å ö 7=Ûü…¿[Z/˜l‹�nv	)
�¦¯ùzèá,‰ãÿ·ÁÄÈÐêù$SÝí&ZŒÎ
-j—µÌ…\¾Ö¬´c7  ^ ‚ ‰ è 
0w€‡Ã(Æß^ªëén³`ƒ½»ÃÌ

š”)}× 4¬÷I¹ð@GŠ�¿jw±HsŽ9 ý �Ag·ŠG	"	'	,	8	=	­	±	

.
x
‰
—
ã
î
.P·½ÉÕäè&ÉÏö-9KT^lú·mbl© ´ á oz0„¨rG	=¡ò�í¾J è˜Ð“™½åzô;){ÙPx�ç0J� o¤±C M Ä ­s‹Íì<}ÁÆQ	T	d	†	‰	Œ	�	’	ª	ô	 
K
’
Â
Ñ
Ô
é
¬±ò36EHVÞäAewz"%Š–«Æç†à.X…‹oÅ´œKa& pÊk¹Â=IÇÜ�½Þž1	B	�	
:žOY{]�®´›§ÂRÍ"ƒŒÇ‚R >²çn	¡	§Ô\eàGƒ®
 +ýºÖA$]y¹Ö lãæmˆHØ7™¾	ä�2Dƒ­Ñjˆ�92N�TNE% Ñ ì ž)ieÏ	c
 
¤
°
¾
L[Îßwo¹¥ó^‘î!‚_­á|¾OEä	 ,õ¶ƒÅ�Sð V£÷	 l¬
DÒýfñ�2-V½¢ u ~¿é(d¯9?fÏÛªß2x÷ecŠÌ›:LSd- á`b‚‘Òô'…¥ïj		g	¦	¨	æ	
¼
È
ç
ì
'vÓ1TÍ724–ÔÃû@Šw ùÔö¬ñ©ÿ
^ƒ»o½sBË(Ò1 ' Ç (*�i	¹	Á	Ö	<
Ê
Þ
ú
*¢C€5€–ÃÔõ"Õw•OÄA e ƒÕÚ 5Ž¥ÄDc‡“§Õ†’ È%G	Ê	Û	Þ	á	è	ë	ï	¹
ÜBµ¸á
èÿ6S˜Gg¸ô÷ç2æ©'\åyŒ$ n h¢¹âcïOØû	
iõ…çYi06žg’Ü;w„ÅéQbu>!€ V ƒþ_	&wB÷ªô+J¯6>~˜9D¢ÍÃƒ Wx,³k”f}âÅã{þyïÔÑ)  4 $Qx§wø`1KÝúW	`'«¢&?[btw@�è„eh6!«¶ºiì÷
" ¥ ÈPvTà9WœÉêM}�×ò"4@Eúq9ú2T}2¸ µC—ÚÁh†R+©œ)3 #õA­Ëâ5Ö[	YÐMÜ�®gk ¯Šû®–†¶‰ãþL Ê3EÛ*v.ì \ª”Öüÿ\˜�–î8VÜ	˜Lé§ž‰“á 3U\qÀú6>’Z(Ë:ô~[  7«½"M¤ÙNdÒ@Ë†^6e7˜y) Oš�Ùa:pO¦}Œ¿ÏxÛt¿Îæ1O‚•¨hÕµ ¢&6oëB
•ú Ï Î^£ªªBÒ
R‹“MH a#8
š6ðJÚf)|  ¹ $�€† ¶n¤ )oî×ó_lºÝïà. E Ì  ÑòÕÝû%F6LÌÒóÐ’½ëiÀáäÿq°|¯uæ	›>ÙŸ¶™è<  ? P ñ *BªÆíÎöüs�ï1[šöuÆ‰J­k±½|…ù´êöæO`²D UÌßŽ"W_®Îoþanr"   Ü M,±�ù	ý	¥ûÍÓÆª¼q«	Òµ¿Ù(ÜsúC;ï$ ! - ˜ IþBXÁ
:Rˆ2
Z
×
Kœ2Éd�ìÈo—�‡¤Ö(Æ0 1 a •   É —²Mû°»	Ã	`
Ì
à
7Iƒ�Dˆ
Þî•˜ã:‚H~=ˆŸºåR·Ô’$ÒÙ~Øñ =G{²C/Åò—ÊEÐ¼¹ �’‹¶ÚÞ3/JÙ­à –Á+‘—	>\V6,ó J ,ºp€¨Ã,bŒp¦¤ìÂ•±·jZ; 7 � zŽßns>
N
l
¢�¢&bl=Ÿô[ [4Øvb‹å*I¹û=ï˜*W
jý¿'  ) ¯ 3Êãç�Ôkx	U
‚
ó
j�¡xÏ’3A¼óù¬Å^eÀÞˆýeB \ k x ‡ » ¿ !9?f‹Md€ƒŸ)JTY�X–·É@j³Ç	%
´‹ Hh›ž#GJwzÔ=¯%PÆëõ;E\ˆ•½´ê³b®¦ôa Â &Ti·ØÿkBÄ5ä	•
Ú$+eð7™zQâ v\Í.dì’‚¥7ÉÐàRy‰—ß,“¶Y5   H … ‘ “ ­ Ú ø RÔÝ9;KôRWV¸÷	Ã"}†€ê#(!1©½9ê÷U²Ò×‚×ÿûY´_G  h | � ç ú 	<iv†ÂÖû'‡¶]
m¢²_‚ºÂËk	Ø		
(
+
u
Å
ü
™Â$®(+ÀÃÏé#«\¸ïý/´v„°÷GŸèl«¶* ³ ü Žç¬/§ëq‰
ó	-
w
Ð
°ã<Dãv!OkªæùwŒœ/I›½ n` koÉÁHÆÛG€×æm	�¦1[šiœMŒÆ¸Q t ë ¢¾öô'8eÎdÎ	ÄÞöò�^‰¬ïKF & d ‚à4�Ñó&’¤«Ôî)i…‘ŸFÕ	Ú	Ý	à	ê	î	
»
Ç
þ
&]·º6ç1)R•¼F·óöræ1Bú•¨!‰äÑvÃ0&  U Pg¸áîNJV	&+hs²ö*I®|ƒÛ5vèaâx¡µ¹îÐ	 ¤ ´‰•ÊÕZ	–8ÉX…DùLùDu1ë" NØN[£¿Ø—¾c�í?wUs�=K…‘´ÿ¡9nê5’	+ ~ Î ÐœÔ`ÍÜ"7¢5K…ÿ©Ï
ê™¿ãþ
5
Im£nåšž^k’{ß:    > ` H–ÅìýWÀ0™õtˆIŽ°ú{Òø³é©»â_}<ÑëT‡ž´Þ'n�QŒ­¶Ín†‘£B× +•±‘*o–	§Â=ÙÉÏIÁ¬¸9 [ j Œ ¾ 28>eyŠ3×•¶?imŒaw	Æ	$
k
�
iŽÿGš¡Šœä¸Åú%î@»òk”¼«³)é]¿­d�8 G g { „ Ù æ ÷ …ÓÜ8†µQ\h×þ[	¡±4^Êã	*
˜Â!*…- "6¿Âè0¨[é.˜yçµ2 ê �½õ�È«ßÐê%Fp‘ªå	(c„žEÍ	Ô	í	ò	Ï
%œ¯; ñ0Z”�›‹ˆALHJ_0 T £ ³à_Ìí„Z”¢¾þU¨½ÎÔ
•*vý€±È<C³ä­øK4uw‘t‘Þê;  _ ƒ ‹ 1x‰Ä*2V”°ýµ]s)É•	#
€
�—Á Ñ÷²º^~ |Ø!5�§ºñHP‹“¬»Àmx�²ŽY  Š é ˆ²¼ô)/01TU“Çìo©´\abœ�'(§¼ÄÅÆÇÈÍÒÓÌ	Ó	ñ	$‰Š‹Œ”•–›9:¿À/~ŽÇŸz{²âãJ‡‘’«ºklIv��  3^  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                      0                                                                                                                                                                                                                                                                                                               F                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                       	                                                                                /                           *                                                                                             +                                                                                                     	                      	                                           #         *   )                                 `   <                  	      	   
            
         	                                             6                  -   F   H         7         /   *      ¥   T            8   ,      ^                   0                                                                                                                                             F                                                                                                                                                                                                                                                         
                                                 	                              /         *                                   +                                   	        	               #   * )           ` <      	  	 
    
   	               6      - F H   7   / *  ¥ T    8 ,  ^   |o  ~  ~=  ~�  ƒ›  Š‹  ’=Á3?=ÛÛÌ óg Ù ê ë VÇÈà\¬ÐNoZ�
&¯°ã¦ÁÂ&G?ò•ó=´Ñ‡Û45PQuÀï•¬Eiÿ°tï«¯h«ä�´N"_�ƒýD¼Ümz»!ÿò_2g \ âJyËa�¼îòûƒýDü	P	…	ˆ	‹	 	§	Â	ç	
ˆ
¯
¸
Ö
Ý
ß
â
ò
 	-³ÔÙ8AJS_Ýõ '¤àí"Nž2?�Öü8¼X55½OùÔöE�½³£ÔöE½ÔöE½½�ëiÿ°1uBáHè,PÉS€^œËÏ %!%ƒ,n0 áîø,? 0B!‚Bô-¸Iu¤©»´�J8B 	C0ôÚó D�d|
}
û=M™¶·=W°ñ àí˜v
g˜gó =CJ“¼çÙ¨Ý0â² .&;†ô(Œ�á{ ý³ßrÕ	Þ >â¾a	
�
+ Øð2}–Uo—ãÎ$ÀÝÝá‡ÓÆÜk Ÿ#ôÏ çŒ=Ù¨Ý0³ßýb:
Ü
èv.œ¶ sj§¬�åå�Èi·•
œ$+�}
5õ±ÏF�ŽâÐ	}
!sôíå2U—â ¼KPs�h"¤r
}
¿×!zâŒ³ßðv ~‹þž-U*±w1 ¨=åÂÖ•;Å Œa:
·
Ü
@³ß~Áotp â ó -Dk�‘¼ÐŒæ!,�á{s
}
Ã
 Àý7¢³ß&KrŒEP=>Éët�pšöæ¶$Ÿl
¢¢=7R¶¹��Ï �  > ¤ Äg�¸àá±Ííî�No‰s—ñ	&:*6°k÷š²é^TÏku¬m ² .Nv›.m€dË6æCâuåvš„		f	¥	3¢^ýž(
+À}o)Íý÷_3É �Þ}{äo¹NõÅ¿?—¶ s�h"jŽâ¤Ð	r
}
!§¿×!sôí¬�å—�è¤ ±—Ïk… KRWV#9_O •FÏî$„�IúþƒD		f	¥	¬	ì	
Ÿ
½
â
KÙCXÈâ3Èé]d5C‚p1¸Í�—&6©“þè�W â ¼o;KBt1PNY¯»»Ìü¹¦Œ Óï��&6"ÂÕ‰E	Ù	QE¶0§ãsŠšÓÆöæÜk Ÿ¶$#Ÿôte6• •ã~Ù$á‡|ú6>âGFšöâf‘·8Öœïå² ©¡j¸.œ~ù?í—Ð&¶<ž S� jˆ‹CÙCâw�)&6!ÃÅÚ™ÅÂ Äi·1 =åâi·l
¢¢7=R¶l*¿O¯èz°åÄXyú(ÍŒó€¾³Yã~â ¼KüPN¯»X ã ½,<LC2QÛZ¼ŒÍ > Åìg¸áî0N™õtˆI	°{øh³é_TÞ�Ûv­ÍèantÀ@ËèÍ��3 Ÿ È #±vL÷_ùV	º	Â	_
Ë
ß
6H‚_C	í>Za”—9G?¹äç#dg5}ë R ô El�‘Ñäe®ñ1ŸÎë‚C“	!
}
~
­7üûŒß$>NtÃÔ²ü �Ž«êp‰	ò	-
w
Ï
¯ã; kù‹H _™¡
cQž¶7¥›$D ŸEËÑh=Øµ˜ô lÑ1Ÿëß$>Ô²3 #v÷_ùV	_>Za?çdg5ëŸ È ±Lº	Â	_
Ë
ß
6H‚C	í”—9G¹ä#}NØN¾ws�´ÿ¡nê	` þBL Š
J
«@‰•Å…ß-WŠ³ódÝ% 3 c Ÿ È #±vL�ò÷_|ÀùP	S	V	c	…	ˆ	‹	Ž	‘	©	º	Â	Ü	ß	é	_
‘
º
Á
Ë
Ó
ß
è
6H‚ñ25GU\_¶¹ÝCy 	$0í(>Za”—õ9G?nq¹Ääçåù”#dg5}ÐëJ/ > ü Åìg�Ž¸á«îê0Np‰™õ	tˆI	ò	-
w
Ï
¯ã;° k{øh³éù_T‹Þ�Ûv­ÍèanH _ ™`¥¾Í0§%Aù© »~`m!V+²|âxîÐ¨ _p}ÙxæÕµ 6ë
v.’‚àyÜ ±¥ª¼qµ(;ÐÐg
‡=P‘š$Åê“H?�¡Ï&7Í9‡yó:îÄý Ü [v±¥ª¼.q¹3’µ(J‚Ùày;,”{yÔ†o×_lï¿	ýÁ3u}c®>wbËc�ßÐ%‘ª(„žEÔ	í	%”A@¶	!	&	+	7	<	¬	°	

ˆ
â
í
-O¼Ôç
ÈÎõ8]§µ	ög@Elãæ(dˆ¯9fØÛ¾	x�ƒˆ2LS Û jnL°^	l	ø	Aªñ¾=‹ìrù·Â:[ j ¾ 8>eŠ•¶?iÆ	$
ÿGšÅ”¼³é­èxê]ÈDôu¿À$C 
šDÐmÄ
rï¦ƒ2qå K…Úð(Ww †  Lc‚SXŒW�Ivy$:²¥ó`µ	g@EC 
šDÐrï¦ƒK§ö% c �ò|ÀP	S	c	…	ˆ	‹	Ž	‘	©	Ü	ß	é	‘
º
Á
Ó
è
ñ25GU\¶¹Ýy $0(õnqÄåù”ÐJ/†,o”×_l{yÔï9™MqÎS”ÙÉ4cnIs Ãõ]«á`b‚‘Òô'…¥ïj		g	¦	¨	æ	
¼
È
ç
ì
'vÓ1TÍ724–ÔÃû@Šw& d ‚à4�Ñó&’¤«Ôî)i…‘ŸFÕ	Ú	Ý	à	ê	î	
»
Ç
þ
&]·º6ç1)R•¼F·óöræ1Bú•¨!‰äÑvÃ0à y—@ƒ’˜¶¼ä|À	!	&	+	7	<	F	P	S	c	…	ˆ	‹	Ž	‘	©	¬	°	

ˆ
‘
Á
Ó
â
è
í
(-O¼Ôçñ
25GU ÈÎØÝõ8]y$nÄ�£J¹ Åö	"YzÆû)5 %ItyÌHK¬bƒËìÄôcG�`¸étÎüýj©Æ Ôa�Óõb„†¦ð			f	h	¥	§	ç	;
¸
É
Ý
æ
ë
ù
uÒ0AS‚´Ìà35n—Ó¡Â&ÔAÊ'x=G{²C/Åò—ÊEÐ¼¹X ã ½p,Ê.<LC×ýu2QÛOZb°¼%zŒº¼Íñ < R Fc”‘åf®òÎÙñ3~‚C“	!
~
­7ý—ûŒVp½˜zäNøªtÄÏ³ ü Žç¬/§ëq‰
ó	-
w
Ð
°ã<Dãv!OkªæùwŒœ/I›½ n`\ª”Öüÿ\˜�–î8VÜ	˜Lé§ž‰“á 5 < N R Fc”ÈÎ%IRty‘ÌåòHf®µÊòKiÎÙíñ#3~ÂÇ2LX‚‹C¥X	“	õ	!
0
L
^
i
s
~
“
Ã
Õ
ñ
5G�­²ÀØ"7I[a¤ý(0Bf{ƒ¬&bƒ—Ëìû'`ÄôcŒ8MVb�´FZxšôAGp�°½G`˜¸Æé7z�…•äNZs†°ÐøtÎüiªµàýht�·»Ä`j©Ï\—ªÚ.3:D”ÖüÿU\^`q¸ÀQ\d˜|	˜	n�–î8VÜ	�˜úü68>@L]np’µé,KUZak{R(§WËˆžæèê:]‰“áçôG~[Ð �èÚ©µÞ1‚«¸�dŒ,¡2 Ê ˜Nú¤ø„�ß`4Î>}æ0Ùšòæ¡B×î6
%ŠÁÞÞ„NâÆø÷ˆG g { „ Ù æ ÷ …ÓÜ8†µQ\h×þ[	¡±4^Êã	*
˜Â!*…- "6¿Âè0¨[é.˜yçµ~…¢yßœçŸ2	C	K	‚	o
{
;+P~±ú^¯µœ¨ÛSc¥NYqýž¡|@„eë#6ìD
Ñ‚†�ÃÈÖöŠ5 F o Ð Ò%7ItyÌÖHP�èKx °Úàôz·©µÞ1`‚ «L	
ö¬¸bƒ�ÁËìd�Äô6c¨1FUd¦;G�ç/6`†³¸é?mÒÝAt¢ÎÚíü…èýŒ·,j©¡j¸*<l±Ý6�Nã€¦ÁÐQ81Õ  0Å     ‡  ‘        ‘Ž®áôÚîÿ€ø�:¸¸Ó¢€·îpàýëøóï­
òëï{û¯ú;¸â(ãõ;«Á7¯®Ã8ßr¾á0o¿È¸îð6îûû_ö<ºÞÎ¿ªúï® ø�nì«¤>»Ç.þŒ¿>ÿ®‰�¶ß*®_õIÿ.þ¤÷îöû£«¨øÿ+¾ kß»{êÚ€Ó¿çÿéû¹.#“ºôÿâØ6ëÐÄNÓ’ë¯½þb÷­MŠŽ>ôø‹üN÷éšýÂ– î
à'ªn/ûß÷HYzÉ“giœR™N¸~ëºû÷@ûƒLDºåØÂ�[ðþC±|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?ønDÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹€»¡¦‡’šY%µ§ ¶’¶OÙ˜¼Êv^œ%`üø8|ýqÿÏÚµ–˜¯Ù%ÏYêö‡-` I©ÂW�ÞƒIÛR&Ù�Rsÿšµ‡	X?>¦?IØ~˜«ç Òvç&’Û9§Ø,BýÊã­îÿnÿÿ¿ßýj‰©©ª¥¦¨�1€   3= À1 À0CB0ÃO[DÃ3¼T«ê˜10Ã?™U1LÌ00³Œs�¬ÿ#üï<óìÏ??Ã¸x|Ê{÷BÃúï¼%¤Î‚¥‡Šº® ã!Sè@;À@þ¢0 L; ÈÈˆˆÈ3ÌŒ;ÌãðÃˆLã0Ì¨Œï4ˆÌ@0ðÿ0Œ Œ@T8ÈÀ"#  !4 ˆË�ÿ�ÿûüJ8MÃðë0òpƒ0Ï#ÀÀ4 < óì @ì @3?ðLðÃ‹Ì1<ÓÚ>©‚[#4¯¨¿Vâ0O¸3Ï7ÿ¹03 Å0Œ3ÃAAXë#,ó<òÿ®þïO°/0 pãë À4°ëƒ";‘>ã—Ø­�®º«çNør8)¢8»mªQbÇÏ}†//‡<ãŽ0‡C°ü0ïÒ<ÌõÚÊ ÂP1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ôs!k{íÐãæÑB8"XþÝY÷„nªþãÕZ`m…?èM¸zàå•jj¶�dˆ Zª"îÞÁAÙ€@Õ¶¥]´XI¨*D¨5Ù�ÙŽ=…Õ
eD-´™…tÿã‹ÚúkÙO¡èB‘ªªf¨ª"šªº—oåZ¯§b©äôj¯¾ï‡žý-“‹û®é�û�?S¦^²dÙÚg¦0ó|&6¥¥´êyÐh!ÑTÿo¤>ûëä»ášåÿß¾)únwö¯�=»»èô_¹B£§Ño€@Dè4 €@P È~?
í ÷øÎ»ô_„ã¸s.Ê·†ø#�:úw¯ÔAÒ Uu[E” ¾¹zƒºß«’ÿZ™¢¾ò2Ò©ø„eI=?ËàÐ8‚@/"^þ¢`Œ¾B+} óî{ú»«½¾º¾•ÿHæàšûÏÑ4ó¯åã¸¸
„¾ƒSôí¿‹ÿ¯²÷Û¶?àîøÿ¯ç@+àî;”ZÏA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjm…¬þ      ñ    �   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "l<?xml version="1.0"?><Features recycledIDs="0" nextValidID="18"><Feature recentID="17" name="EGFP" directionality="1" translationMW="26810.33" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="4467" maxFusedRunOn="4467"><Segment name="1a" range="3667-3669" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3670-4380" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="COX8 presequence" directionality="1" translationMW="2703.34" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3645" consecutiveTranslationNumbering="1" maxRunOn="4467" maxFusedRunOn="4467" detectionMode="exactProteinMatch"><Segment range="3571-3645" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;mitochondrial presequence of human cytochrome &lt;i&gt;c&lt;/i&gt; oxidase subunit VIII (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/2543673&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/2543673&quot;&gt;Rizutto et al., 1989&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7447" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6656-7447" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7448-7516" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="958-1557" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5897-6485" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3562-3570" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1825-2204" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="chicken Î²-actin promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2206-2483" color="#ffffff" type="standard"/></Feature><Feature recentID="9" name="chimeric intron" type="intron" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3502" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;chimera between introns from chicken Î²-actin and rabbit Î²-globin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1595-1819" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="Î²-globin poly(A) signal" type="polyA_site" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4535-4590" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;rabbit&amp;nbsp;Î²-globin polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="HA-L" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-804" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human &lt;i&gt;PPP1R12C&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="HA-R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5013-5849" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human &lt;i&gt;PPP1R12C&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="945" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="1557" maxFusedRunOn="1557" detectionMode="exactProteinMatch"><Segment range="895-948" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="806-839" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4939-4972" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="846-871" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;splice acceptor site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   Æ<?xml version="1.0"?><Primers recycledIDs="2,8,13,4,1,0,7,6,5,9,10,3,11,12" nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  è<Notes>
<UUID>c93c1ddd-4fd9-402b-9882-f0cfdec590fb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pAAVS1-PC-GCaMP6f</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>GCaMP6f knock in construct into the AAVS1 locus.</Description>
<Created UTC="16:0:0">2018.1.9</Created>
<LastModified UTC="6:7:43">2024.9.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference journal="Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10." pubMedID="26971820" title="CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs." authors="Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR"/>
</References>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="3570..4379" colors="green"/></TopStrand><BottomStrand><ColorRange range="3570..4379" colors="green"/></BottomStrand></StrandColors>  1t     ‡  ’        ’Ž®áôÚîÿ€ø�:¸¸Ó¢€·îpàýëøóï­
òëï{û¯ú;¸â(ãõ;«Á7¯®Ã8ßr¾á0o¿È¸îð6îûû_ö<ºÞÎ¿ªúï® ø�nì«¤>»Ç.þŒ¿>ÿ®‰�¶ß*®_õIÿ.þ¤÷îöû£«¨øÿ+¾ kß»{êÚ€Ó¿çÿéû¹.#“ºôÿâØ6ëÐÄNÓ’ë¯½þb÷­MŠŽ>ôø‹üN÷éšýÂ– î
à'ªn/ûß÷HYzÉ“giœR™N¸~ëºû÷@ûƒLDºåØÂ�[ðþC±|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?ønDÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹€»¡¦‡’šY%µ§ ¶’¶OÙ˜¼Êv^œ%`üø8|ýqÿÏÚµ–˜¯Ù%ÏYêö‡-` I©ÂW�ÞƒIÛR&Ù�Rsÿšµ‡	X?>¦?IØ~˜«ç Òvç&’Û9§Ø,BýÊã­îÿnÿÿ¿ßýj‰©©ª¥¦¨�1€   3= À1 À0CB0ÃO[DÃ3¼T«ê˜10Ã?™U1LÌ00³Œs�¬ÿ#üï<óìÏ??Ã¸x|Ê{÷BÃúï¼%¤Î‚¥‡Šº® ã!Sè@;À@þ¢0 L; ÈÈˆˆÈ3ÌŒ;ÌãðÃˆLã0Ì¨Œï4ˆÌ@0ðÿ0Œ Œ@T8ÈÀ"#  !4 ˆË�ÿ�ÿûüJ8MÃðë0òpƒ0Ï#ÀÀ4 < óì @ì @3?ðLðÃ‹Ì1<ÓÚ>©‚[#4¯¨¿Vâ0O¸3Ï7ÿ¹03 Å0Œ3ÃAAXë#,ó<òÿ®þïO°/0 pãë À4°ëƒ";‘>ã—Ø­�®º«çNør8)¢8»mªQbÇÏ}�‹ËáÏ8ãŒ¡Ðì?;ô�3=Fö²€ð„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�ÄâuÈZÞûB48ù´P‚ƒÖ?ƒ·V}áª¿¸õV˜ÛaOún¸9eAZš­£Y"(ªˆ»·°Pv`5m©Wmj
‘*vcöcƒ�auBƒ™Qm&a]?øâö¾šöESèz¤jª™ª*ˆ¦ª®¥ÛùV«éØªy=«ï»áç¿Kdâþëº`FþÇãOÔé—¬™6v™Å)Œ<ß	�©iFm:ž@ttU?Ûé¾úù.øf€¹÷ïŠCF¾›‚ßý«àOnîú=îP¨éÆôDà:   2�Â»H=þ3Ÿîýá8îÃ‹‚2­á¾ãN¾�ëõE4¨]VÑe(/„®^ î·êä¿Ö¦h¯¼Œ´ª~!CÒOOòø4 „ ‹È�W¿¨„X#/�ŠßBÈ<ûžþ®êïo®¯¥D¿Ò9¸f¾óôMüëùCøî®¡/ ÔýC;C¯âÿëì½öí�ø;¾?ëùÐ
ø;Žå³Ð_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¡k?
      ò    ‘   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="EGFP" directionality="1" translationMW="26810.33" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="4468" maxFusedRunOn="4468"><Segment name="1a" range="3668-3670" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3671-4381" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="COX8 presequence" directionality="1" translationMW="2703.34" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3646" consecutiveTranslationNumbering="1" maxRunOn="4468" maxFusedRunOn="4468" detectionMode="exactProteinMatch"><Segment range="3572-3646" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;mitochondrial presequence of human cytochrome &lt;i&gt;c&lt;/i&gt; oxidase subunit VIII (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/2543673&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/2543673&quot;&gt;Rizutto et al., 1989&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7448" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6657-7448" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7449-7517" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="958-1557" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5898-6486" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3562-3571" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1825-2204" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="chicken Î²-actin promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2206-2483" color="#ffffff" type="standard"/></Feature><Feature recentID="9" name="chimeric intron" type="intron" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3502" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;chimera between introns from chicken Î²-actin and rabbit Î²-globin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1595-1819" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="Î²-globin poly(A) signal" type="polyA_site" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4536-4591" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;rabbit&amp;nbsp;Î²-globin polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="HA-L" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-804" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human &lt;i&gt;PPP1R12C&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="HA-R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5014-5850" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human &lt;i&gt;PPP1R12C&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="945" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="1557" maxFusedRunOn="1557" detectionMode="exactProteinMatch"><Segment range="895-948" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="806-839" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4940-4973" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="846-871" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;splice acceptor site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Feature 17" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4382-4406" color="#ffff00" type="standard"/></Feature></Features>
   Æ<?xml version="1.0"?><Primers recycledIDs="2,8,13,4,1,0,7,6,5,9,10,3,11,12" nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  è<Notes>
<UUID>c93c1ddd-4fd9-402b-9882-f0cfdec590fb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pAAVS1-PC-GCaMP6f</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>GCaMP6f knock in construct into the AAVS1 locus.</Description>
<Created UTC="16:0:0">2018.1.9</Created>
<LastModified UTC="6:7:21">2024.9.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference journal="Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10." pubMedID="26971820" title="CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs." authors="Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR"/>
</References>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="3571..4380" colors="green"/></TopStrand><BottomStrand><ColorRange range="3571..4380" colors="green"/></BottomStrand></StrandColors>  -q      ì  G        GŽ®áôÚîÿ€ø�:¸¸Ó¢€·îpàýëøóï­
òëï{û¯ú;¸â(ãõ;«Á7¯®Ã8ßr¾á0o¿È¸îð6îûû_ö<ºÞÎ¿ªúï® ø�nì«¤>»Ç.þŒ¿>ÿ®‰�¶ß*®_õIÿ.þ¤÷îöû£«¨øÿ+¾ kß»{êÚ€Ó¿çÿéû¹.#“ºôÿâØ6ëÐÄNÓ’ë¯½þb÷­MŠŽ>ôø‹üN÷éšýÂ– î
à'ªn/ûß÷HYzÉ“giœR™N¸~ëºû÷@ûƒLDºåØÂ�[ðþC±|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?ønDÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹€»¡¦‡’šY%µ§ ¶’¶OÙ˜¼Êv^œ%`üø8|ýqÿÏÚµ–˜¯Ù%ÏYêö‡-` I©ÂW�ÞƒIÛR&Ù�Rsÿšµ‡	X?>¦?IØ~˜«ç Òvç&’Û9§Ø,BýÊã­îÿnÿÿ¿ßýj‰©©ª¥¦¨�1€   3= À1 À0CB0ÃO[DÃ3¼T«ê˜10Ã?™U1LÌ00³Œs�¬ÿ#üï<óìÏ??Ã¸x|Ê{÷BÃúï¼%¤Î‚¥‡Šº® ã!Sè@;À@þ¢0 L; ÈÈˆˆÈ3ÌŒ;ÌãðÃˆLã0Ì¨Œï4ˆÌ@0ðÿ0Œ Œ@T8ÈÀ"#  !4 ˆË�ÿ�ÿûüJ8MÃðë0òpƒ0Ï#ÀÀ4 < óì @ì @3?ðLðÃ‹Ì1<ÓÚ>©‚[#4¯¨¿Vâ0O¸3Ï7ÿ¹03 Å0Œ3ÃAAXë#,ó<òÿ®þïO°/0 pãë À4°ëƒ";‘>ã—Ø­�®º«çNør8)¢8»mªQbÇÏ}‚‡¶Ü²R”t-Í-™·±.fÔ<u4QpÛPÅëQ¼÷]±24ìù÷ŸMöÃ?Èã�Ç_yøL�/W«õÄS1·`¾8²<ð{°ØN'P0ŸØ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPçoA3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×ãÁõááDÑ³E©Q®þjAÀYuõàc¸EÿtSDãAØmaIñÂ7[¿„¯‡X`ÔUaYÐÁE¤E2°ˆ¨e`çm#M¬÷!‡Wè¥Ñ…¡…a´4vÆ `´%yÅ¢ua‡LR2·¾Ð�>m ƒ‚ õ�àíÕŸxFê¯î=U¦ vØSþ„Û‡®YPV¦«hÖHŠª¢.íì˜[jUÛE„š‚¤Jƒ]˜ý˜àãØ]P æTBÛI˜WOþ8½¯¦½‘Tú„)ª¦jŠ¢)ª«©vþUªúv*žOFªûîøyïÒÙ8¿ºî˜¿±øÓõ:eë&M�¦qJc7ÂcjZQ›N§�‚OöúCï¾¾K¾ ._ýûâ�Ñ¯¦à‡wÿjøÛ»¾�Eû”*:q�=øNƒ@ Œ‡ãð®ÒŒçû¿EøN;‡0âàŒ«xo‚8Ó¯§zýDM*WU´YJá+—¨;­ú¹/õ©š+ï#-*ŸˆFPô“Óü¾ˆ! "ò Uïê!Ëä"·Ð²>ç¿«º»Ûë«éQ/ôŽnÙ¯¼ý@¿:þPþ;€ë€¨Kè5?AÎÐëø¿úû/}»cþï�úþt¾ã¹@Å¬ôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð(ZÏ:      F   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  g<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" classification="1" name="æ�’å…¥ä½�ç‚¹" geneticCode="-1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4815-4816" color="#ff0000" type="standard"/></Feature><Feature recentID="16" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7885" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7094-7885" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7886-7954" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="958-1557" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6335-6923" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3562-3571" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="12" name="calmodulin-binding peptide" directionality="1" translationMW="2193.55" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4818" maxFusedRunOn="4818" detectionMode="exactProteinMatch"><Segment range="3583-3639" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;calmodulin-binding peptide from avian smooth muscle myosin light chain kinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1825-2204" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="chicken Î²-actin promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2206-2483" color="#ffffff" type="standard"/></Feature><Feature recentID="9" name="chimeric intron" type="intron" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3502" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;chimera between introns from chicken Î²-actin and rabbit Î²-globin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1595-1819" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="Î²-globin poly(A) signal" type="polyA_site" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4973-5028" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;rabbit&amp;nbsp;Î²-globin polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="HA-L" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-804" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human &lt;i&gt;PPP1R12C&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="HA-R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5451-6287" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human &lt;i&gt;PPP1R12C&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="945" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="1557" maxFusedRunOn="1557" detectionMode="exactProteinMatch"><Segment range="895-948" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="806-839" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5377-5410" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="846-871" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;splice acceptor site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Feature 17" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4788-4843" color="#ffff00" type="standard"/></Feature></Features>
   Æ<?xml version="1.0"?><Primers recycledIDs="2,8,13,4,1,0,7,6,5,9,10,3,11,12" nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  è<Notes>
<UUID>c93c1ddd-4fd9-402b-9882-f0cfdec590fb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pAAVS1-PC-GCaMP6f</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>GCaMP6f knock in construct into the AAVS1 locus.</Description>
<Created UTC="16:0:0">2018.1.9</Created>
<LastModified UTC="6:4:56">2024.9.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference journal="Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10." pubMedID="26971820" title="CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs." authors="Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR"/>
</References>
</Notes>
  ý7zXZ  æÖ´F !   t/å£àh¼
Ì] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÃ2"~˜àY§Íú%c;§bOuüI¸š‹i²<ŠÒDÖ•lJÁ-sä®‰±ãŸ7 ŸÔ;E'Ãÿ‡‘[Í¬vJ—åùMj„%“j–nÚ'OÖçãš�@
z\îÏŽõ¢7!]QÅ>ADAøc¢�tíÃ·—§¦,ä›Ï|/’Øõ‚¤×ü	Ä‹10ÆiJèÐdž&(å”kf¦Ðhµ_ñE(_mF3¶wŒEþˆÔZåÄùÃ´Ð�ðÀÏ¤vÊS‚°ò®´õÏ~ƒcs"°Îw¸&˜ôÝpös®åõÈæ�Ã;x�]/¢­ë/ib³	¹ãaMúÇJÒ®èEÝØ[nfpÕODPâ›qÈ>Ðêóê*Ùó`7�JÓ�ìËPÕE3HŸ�Þ¸ÇiŒÀzžKßù™›v|~¸ù°M8 ¶¯\ûŠàyê„ÐËv7]IÓ›b¸©¿?~€£Jc@©ãfw,©/¡}œñ¿r£6Ù.aïà»ªØƒÎá˜›ÂS|^ÑÙÃ¦1Õï8&ñqlÞÛ­†¹Å–’*Oø®r‹êuhñƒ¶z<~«
é…d¯#*‚þb'Æû!¼¯søù$Vb¶$ÞFJNG”8�'šE˜’á‡Çëb4Á¡‰WdyAo‘/rÁšÃ‚»nnuWþŽ" *<éå�™ÿzðÕw‰Ó4€Y5d ŽÑÀè¶��²�ü «hÂžó#’öÎ1œ=ç|”"Ð—ÑÅYsmPè€#*…|ŠO©eßùc£ÉA»ò*fßâX¦ÏýðÃî1hEªaH>ä×´éVZ5¨ú®\…=�<aWÒ@‡ÖÒRšÕJ:9h{oÿuªRVeÚ‹äý‹�Àvö‚Ä°wBÇ“jË-ƒÊÒ0oæSÞýÝ2w‘:ëQ—õKÍ:?Ž{9ÀŒ|´ƒÊß³€�m°¯—9«[´%’R˜5Dg{°™Y’�|_‚38oh°˜ÿÑd1ÔÌ×S÷Ã$Ûa€Ó§t\4VóJFsI´Ú	:1XšŸ œæ¹2nöl3kòIo§ OFJX.uCI|â:á—¿]ßóÃZ91F*Uû'Ê`»5.‹Ýw3ýwêæ2Ñ*½HP=Ïü´á¢§F ±¯ÿ`µÈ¡‚.~?gD±§ôKÂ¯gRpõÄÆ›úrÖ·Ò�žc3ÿ¢¡°´Ë ¨9¿Æ—;Žºjb›
�*vÚ§±Cníè½D°¤±6ûY±¹K÷Q8xKQgöŠ”;/]wúÔM¬ýÄGòM[Ò¤0Á-·©tÀTÿà“võ�È0é;4„ÔÈüÖmÀÖbFV·ïÅ¡ƒ±¼õs8-ÚZÁÚÚHÆ*hh±(�S»{<HÏ˜7ŠÈ÷é¶�I-Øª1å7ùS-ÎP� ®Ý7ß°¾ÅÌŽ@ærµuL‡§±‰,Òª’�W,/îÐîTðmµ¡_ÚÏàáœ»M ’fùÛ”¾-pV úà¨*éÅ oVT…æj©7=Ï ©;	‡Û™z_Ì¦…é¢kÉþýr*É4!²Í^duŒn<zI=:53Q6ñ�¼òk<ôk³¬þ1��ó×Øm„-@3[×ÎFûÖ¼¶ß/8ÇˆÕK+6ÿiÄV¦Ã[&Þy/z¯*)£ø‘b°‚,q6Œ4à<,=Å#!1X!÷Ž
&•Â’%O‘Ð8·nâcö‡…ø´Û{ÑÂ|0´P1Éþ¨õ€ï‡¤ŠÁJ.Š'“3/�Ôç’bÁ Óc¥A}Ò�M~'jÓkíŸyÃ-ûÛe<Ö8ÅöJÉK"Æ7Ì,

˜æsžÒ3�‚TîèT‘A`•‹è>¥ 7Åâr!†±vfÛ–:ä²?w ÅñÕlj¿ß€AÛ°öôeøKóy‚)	q :²2ºeÄ×,Ó“Üzž¾¨ ñç,k€’Â4!,N?ÂÓ•QÀß2—V.ÒìB{�½ð!<¶÷¾%œ(ÓHŽr’r�¬gøfŠÉ¡ ´Ò,0žšŸa%ú�bAÿ?Rf‘.Ð¾ùI!‚/d¸éô¥€:ßšu~jô'˜Î¹hÇ|Ãá=�…Ç¾)‘‰¶ Öâz”?1uº*Mè¶³jë«±[4†¡û‘'EÉ-S§‹v8ñïèÝJÛañË·EtZ²Œ±Û˜�ù‰!À5*åmŒÛk}SÕÈÇ3H�4ôõŒØ¥%‡åõP’
ý0tÒméMa´3to“²ú³�ô¡¨='«AŒ’M$¡Ô&3¬ˆÁ€Ašf¦ñÔ¬iÕÊáawÂ.�Ç
bÓ˜$ËÊ"s”ã�ª’’ó�re”ÄMîÛ�®éÐëc'È¢í¨OÆ;¢v#iX-<ÃÊf	¬²}áLôù½®×‹¡¿LáÝODSìUbdªórðxýDçÐ’ßœ—]î{;Æ"øšOsõ´éMpÐ6­»ÕÎõKCí†ñN®x<¹VÙÝï­B§ÄQ{«*ñTQz}£î¡åª2¨ÔB–I±¼ðÒ•A1Ã´d•~1Ùþ×AãËøìËk¯øo�´Y&á„X ÷È_þñQ¡Y/òÊû„�Éß�—3ðª@dGnc	ë6Ó3)[¯ÖüÍ¨Kù¢TÏh¼çœ³Hct$¨lê÷rLoâo^]ûßÁðÐñG�‡æ¢v‰¼YMÎTL·„ñŒ*¼]EÓ?~ÖLŸh æ~N…Ü?d	¦Ô¨û;*ú‚wÃÏÍ|
ÊQ�»"š²Â`Ry%§Æ†Þ7&ˆ§ÈŠ�P£i¯CPŸG�•p5™•ÂvóêÕr¥Í [d;AëÑïø)B9äÈ)ï^ÙîKŸí�…ôŠ~ „ê?^j!Ý2]#ÐßmøxÃ”d¦É\Þ2~ìæ†è­ù’�áP*†€¾Î¿ ']9nÉn$“}T«	× º‚ 'suÀwdÝÑš,nÿ;´'Ô7NK,G_&‚Ú)rœ´ÅÇ\¢3Ûk9I«�Û«ã¤¶b‹5q!elØE…¹©ÓÕP«£:YÞ÷4Î}7ÌW´P{>Ò)%bÿKâWí%u-'×y'*mÞ=3nÇÿ/íÕšà÷¶lu‚×.¼£ëæÄ¬Y�/d.óTø`.d€ R÷¡"B7ƒÊ&¬¡�›ê¥�üšbu"o®®@Z­"#¾¶XÔ<ÝîKg#u_lF¡òå
:20úc#f*C{«Ík�ç›Ygýc§)µ2·»+t"eí€Å*šz\Œ.Ï«™ÚîAj˜þz*M“c—û&‘}¤ínÅ«%�¥äÞþì±Éw/î¯= î-+°Šd÷|ïìMŠhýr›á§ÛB0h{Eäj­5Tªí•®ó$ØñpíZ¼Îÿ†Åoÿi±{œCWðˆÑ“c1w; _5®zöÝÂ=–ogjcdÉOþªÒ&…é $ÍÄíðÑ-*QÐ/‡)pzv†¡»
bâß�&®UKÇs!}KkÿH‚´³vëßæ£Àx™3�ƒBÐ°[?½cÅ'­:Q�a2ˆžƒ*þÃÎ,wûøŒañFF÷ÈP S¤˜¯—T"7   ät$#,}’½ è½Ñ Á­
ë±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="0" name="EGFP" directionality="1" translationMW="26810.33" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="4383" maxFusedRunOn="4383"><Segment name="1a" range="3667-3669" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3670-4380" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"/></Q></Feature><Feature recentID="1" name="COX8 presequence" directionality="1" translationMW="2703.34" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3645" consecutiveTranslationNumbering="1" maxRunOn="4383" maxFusedRunOn="4383" detectionMode="exactProteinMatch"><Segment range="3571-3645" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;mitochondrial presequence of human cytochrome &lt;i&gt;c&lt;/i&gt; oxidase subunit VIII (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/2543673&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/2543673&quot;&gt;Rizutto et al., 1989&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSVLTPLLLRGLTGSARRLPVPRAK"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7449" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6658-7449" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7450-7518" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="958-1557" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5899-6487" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3562-3570" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="6" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1825-2204" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="chicken Î²-actin promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2206-2483" color="#ffffff" type="standard"/></Feature><Feature recentID="8" name="chimeric intron" type="intron" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3502" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;chimera between introns from chicken Î²-actin and rabbit Î²-globin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1595-1819" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="Î²-globin poly(A) signal" type="polyA_site" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4537-4592" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;rabbit&amp;nbsp;Î²-globin polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="HA-L" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-804" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human &lt;i&gt;PPP1R12C&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HA-R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5015-5851" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human &lt;i&gt;PPP1R12C&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="945" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="1557" maxFusedRunOn="1557" detectionMode="exactProteinMatch"><Segment range="895-948" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="14" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="806-839" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4941-4974" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="846-871" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;splice acceptor site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  w<Notes>
<UUID>c93c1ddd-4fd9-402b-9882-f0cfdec590fb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pAAVS1-COX8-EGFP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>GCaMP6f knock in construct into the AAVS1 locus.</Description>
<Created UTC="16:0:0">2018.1.9</Created>
<LastModified UTC="7:54:46">2025.11.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference type="Journal Article" volume="18" title="CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs." journalName="Cell Stem Cell" doi="10.1016/j.stem.2016.01.022" date="2016-04-7- 7" issue="4" journal="Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10." authors="Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR" pubMedID="26971820" pages="541-53"/>
</References>
</Notes>
  Y     –         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                             7<StrandColors><TopStrand><ColorRange range="0..3559" colors="black"/><ColorRange range="3560..3572" colors="light blue"/><ColorRange range="3573..4379" colors="orange"/><ColorRange range="4380..4381" colors="light blue"/><ColorRange range="4382..7570" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..3559" colors="black"/><ColorRange range="3560..3572" colors="light blue"/><ColorRange range="3573..4379" colors="orange"/><ColorRange range="4380..4381" colors="light blue"/><ColorRange range="4382..7570" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
